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Catalog Number | orb313093 |
---|---|
Category | Antibodies |
Description | SLC32A1 Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Bovine, Human, Porcine, Rabbit |
Reactivity | Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human SLC30A9 (351-450/525aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 57 kDa |
Target | SLC32A1 |
UniProt ID | Q9H598 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | bA122O1.1; GABA and glycine transporter; hVIAAT; S Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Sample: Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-SLC32A1 (orb313093) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 57kD, Observed band size: 57kD.
Sample: heart (Mouse) Lysate at 40 ug, Primary: Anti-SLC32A1 (orb313093) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 57kD, Observed band size: 60kD.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-SLC32A1 Polyclonal Antibody, Unconjugated (orb313093) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rat, Yeast | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC, WB | |
Bovine, Canine, Primate | |
Gallus, Mouse, Rat, Xenopus | |
Rabbit | |
Polyclonal | |
Unconjugated |