SLC26A6 Antibody

• Catalog Number: orb1248570
• Category: Antibodies
• Description: SLC26A6 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SAPRTHQWRT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 90kDa band observed in Human renal cortical brush-border membrane lysates (calculated MW of 83.0kDa according to NP_075062.2). Recommended concentration: 10ug/ml for overnight incubation.Immunohistochemistry:In paraffin embedded Human Kidney showsstaining of brushborders along the entire proximal convoluted tubule. Recommended concentration, 0.1-0.3mg/ml. Please note the unusually low dilution required for this type of application!
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 90kDa
• Target: SLC26A6
• UniProt ID: Q9BXS9
• NCBI: NP_599025.2, NP_075062.2, NP_001035544.1, NP_602298.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SLC26A6, solute carrier family 26, member 6, DKFZp
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 90kDa band observed in Human renal cortical brush-border membrane lysates (calculated MW of 83.0kDa according to NP_075062.2). Recommended concentration: 10ug/ml for overnight incubation.Immunohistochemistry:In paraffin embedded Human Kidney showsstaining of brushborders along the entire proximal convoluted tubule. Recommended concentration, 0.1-0.3mg/ml. Please note the unusually low dilution required for this type of application!
• Expiration Date: 12 months from date of receipt.

orb1248570 (0.1 mg/ml) overnight staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, CY3-staining.

orb1248570 (10 ug/ml) staining of Human renal cortical brush-border membrane lysate (90 ug protein denatured at 37C for 30min) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was overnight at 4C. Detected by NBT/BCIP.