SLC12A2/NKCC1 Antibody

• Catalog Number: orb1538680
• Category: Antibodies
• Description: SLC12A2/NKCC1 Antibody
• Target: SLC12A2 / NKCC1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Equine, Gallus, Human, Porcine
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-NALQHSIRLSGVEDH, from the internal region of the protein sequence according to NP_001037.1NP_001243390.1.
• Tested applications: ELISA, IF, IHC, IHC-P
• Dilution range: IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:64000)
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 75kDa band in Human, Mouse and Rat Skeletal Muscle, Heart and Brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 131kDa according to NP_001037.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SLC12A2, Basolateral Na-K-Cl symporter, BSC2, MBSC
• Note: For research use only

Anti-SLC12A2 / NKCC1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

Goat Anti-SLC12A2 Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Goat Anti-SLC12A2 Antibody Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton.