SLC12A2 Antibody

SKU: orb1249189

Description

SLC12A2 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Immunohistochemistry:In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 3.75µg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ vesicles of HeLa cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NALQHSIRLSGVEDH
• Target: SLC12A2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SLC12A2, solute carrier family 12 (sodium/potassium/chloride transporters), member 2, BSC, BSC2, MGC104233, NKCC1, basolateral Na-K-Cl symporter, bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1

orb1249189 (3.75 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

orb1249189 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic/vesicle and membrane staining.

orb1249189 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

SLC12A2

UniProt

P55011

RefSeq (Protein):NP_001243390.1, NP_001037.1