SLC10A2 Rabbit Polyclonal Antibody

SKU: orb1291529

Description

SLC10A2 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Lipid Metabolism, Lipids and Lipoproteins Metabolism, Metabolism

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant mouse SLC10A2 protein (306-348/348aa)
• Target: SLC10A2
• Molecular Weight: 38 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SLC10A2; Apical sodium dependent bile acid transporter; Apical sodium-dependent bile acid transporter; Ileal sodium dependent bile acid transporter; Ileal sodium-dependent bile acid transporter; Ileal sodium/bile acid cotransporter; ISBT; Na+bile acid cotransporter; Na+dependent ileal bile acid transporter; NTCP2; Sodium/taurocholate cotransporting polypeptide, ileal; solute carrier family 10(sodium/bile acid cotransporter family); Solute carrier family 10 member 2.

Measured by its binding ability in a indirect ELISA. Immobilized Mouse SLC10A2 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Mouse SLC10A2 Antibody, the EC50 is 179.9 ng/ml.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SLC10A2) Polyclonal Antibody, Unconjugated (orb1291529) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human LOVO cell lysates, Lane 2: Human SW480 cell lysates, Lane 3: Human 293T cell lysates, Primary: Anti-SLC10A2 (orb1291529) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 38 kDa, Observed band size: 46 kDa.

Quick Database Links

Gene Symbol

SLC10A2

UniProt

P70172