SIRT7 Rabbit Polyclonal Antibody

• Catalog Number: orb100696
• Category: Antibodies
• Description: SIRT7 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Gallus, Human, Porcine, Rat
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SIRT7 (231-330/400aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 45 kDa
• Target: SIRT7
• UniProt ID: Q9NRC8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: NAD dependent deacetylase sirtuin 7; NAD-dependent
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: mouse splenocytes (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µl in 100 µL 1X PBS containing 0.5% BSA (green).

Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-SIRT7 (orb100696) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 45kD, Observed band size: 50kD.

Sample: Small intestine (Mouse) Lysate at 40 ug, Primary: Anti-SIRT7 (orb100696) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 45kD, Observed band size: 50kD.

Tissue/cell: mouse placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-SIRT7 Polyclonal Antibody, Unconjugated (orb100696) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.