SIRT4 Antibody

• Catalog Number: orb1246952
• Category: Antibodies
• Description: SIRT4 Antibody
• Target: SIRT4
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: SRCGELLPLIDPC
• UniProt ID: Q9Y6E7
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 38kDa band observed in Human Kidney lysates (calculated MW of 35.2kDa according to NP_036372.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section. Recommended concentration: 2-3ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SIRT4, SIR2L4, sirtuin (silent mating type informa
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_036372.1

orb1246952 (1 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246952 (2.5 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.