SIRT3 antibody

• Catalog Number: orb19075
• Category: Antibodies
• Description: Goat polyclonal antibody to SIRT3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 43.6; 28.6
• Target: SIRT3
• Entrez: 23410
• Protein Sequence: QRETGKLDGPDK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti SIRT3 antibody, anti sirtuin (silent mating t
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 28kDa band observed in Human Liver lysates (calculated MW of 28.6kDa according to NP_001017524.1). In transfected HEK293 transiently expressing SIRT3 bands of approx. 35kDa and 48kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19075 (0.2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.