Sirt3 Antibody

• Catalog Number: orb1271027
• Category: Antibodies
• Description: Sirt3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Hamster, Mouse
• Isotype: Rabbit Ig
• Immunogen: This Sirt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of Mouse Sirt3.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 37 kDa
• Target: Sirt3
• UniProt ID: Q8R104
• NCBI: Q8R104
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: NAD-dependent protein deacetylase sirtuin-3, 351-,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:64000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + CHO whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of lysate from mouse heart tissue lysate, using Mouse Sirt3 Antibody at 1:1000.

Western blot analysis of Sirt3 Antibody in mouse brain tissue lysates (35 ug/lane)

Western blot analysis of Sirt3 Antibody in CHO cell line lysates (35 ug/lane)

Sirt3 Antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.