SIRT1 antibody

• Catalog Number: orb22540
• Category: Antibodies
• Description: Goat Polyclonal to SIRT1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Dilution range: ELISA: 1:64000, WB: 0.05-0.2 μg/ml, IHC-P: 3-5µg/ml
• Conjugation: Unconjugated
• MW: 81.7; 50.5
• Target: Sirtuin 1 / SIRT1 (aa577-590)
• Entrez: 23411
• Protein Sequence: EKPQEVQTSRNVES
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti sirtuin type 1 antibody, sir2-like 1 antibody
• Note: For research use only
• Application notes: WB: Approx 120kDa band observed in lysates of cell line Jurkat (calculated MW of 81.7kDa according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9.; PMID: 12960381). Recommended concentration: 0.05-0.2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of formalin-fixed and paraffin embedded human Skin tissue using SIRT1 antibody

Western blot analysis of Jurkat cell lysate using SIRT1 antibody (35ug)