SIRT1 antibody

• Catalog Number: orb11372
• Category: Antibodies
• Description: SIRT1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Gallus, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SirtT1 (101-200/747 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=1μg/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 58/81 kDa
• Target: SIRT1
• UniProt ID: Q96EB6
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: SIR2L1; NAD-dependent protein deacetylase sirtuin-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Caruso, R. et al. Defective expression of SIRT1 contributes to sustain inflammatory pathways in the gut Mucosal Immunol, 7, 1467-1479 (2014)

PubMed: 24850427

Applications

WB

Reactivity

Human

Western blot analysis of mouse liver lysates (Lane1), mouse brain lysates (Lane2) dilution at:1:200 using SIRT1 antibody

Immunohistochemical analysis of formalin-fixed and paraffin embedded human endometrial carcinoma tissue (dilution at:1:200) using SIRT1 antibody

IHC-P of human gastric carcinoma tissue (dilution at:1:200) using SIRT1 antibody

Western blot analysis of Mouse skin Lysate using SIRT1 antibody

Western blot analysis of U937 cell Lysate using SIRT1 antibody

Western blot analysis of Hela Cell Lysate using SIRT1 antibody

Western blot analysis of Mouse heart Lysate using SIRT1 antibody

Flow cytometric analysis of MCF-7 cells using SIRT1 antibody

Immunohistochemical staining of human endometrium carcinoma using SIRT1 antibody