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Catalog Number | orb1961731 |
---|---|
Category | Proteins |
Description | Siglec-9 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 90-120 KDa and the accession number is AAH35365.2. |
Tag | C-hFc |
Purity | 98.00% |
Protein Sequence | Gln18-Gly348 |
UniProt ID | AAH35365.2 |
MW | 90-120 KDa (reducing condition) |
Application notes | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | Siglec-9 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 90-120 KDa and the accession number is AAH35365.2. |
Expression Region | Gln18-Gly348 |
Storage | -20°C |
Note | For research use only |
> 95% as determined by Tris-Bis PAGE | |
Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result. |
> 95% by Tris-Bis PAGE | |
Recombinant Human siglec 9 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln18-Gly348) of Human siglec 9 fused with a hFc His Tag tag at the C-terminal. |
Greater than 95% as determined by reducing SDS-PAGE. | |
85-95 KDa (reducing condition) |
98.00% | |
64.13 kDa (predicted); 97.5 kDa (reducing conditions) |