You have no items in your shopping cart.
Cart summary
Siglec-15 Protein, Mouse, Recombinant (His)
Catalog Number: orb1962013
| Catalog Number | orb1962013 |
|---|---|
| Category | Proteins |
| Description | Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO−-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts |
| Tag | C-6xHis |
| Purity | 98.00% |
| MW | 30-40 KDa (reducing condition) |
| UniProt ID | A7E1W8 |
| Protein Sequence | Arg24-Thr262 |
| Expression System | HEK293 Cells |
| Biological Origin | Mouse |
| Biological Activity | Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO−-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts |
| Expression Region | Arg24-Thr262 |
| Storage | -20°C |
| Note | For research use only |
| Application notes | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Expiration Date | 6 months from date of receipt. |
- Item 1 of 1
Human SIGLEC15 Protein, mFc-His Tag [orb689444]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 53.6 kDa after removal of the signal peptide.The apparent molecular mass of SIGLEC15-mFc-His is approximately 55-70 kDa due to glycosylation.
Mammalian
50 μg, 100 μg, 10 μg Recombinant Mouse Siglec-15/CD33L3 Protein with Fc tag, Fc His Tag [orb1550692]
> 90% by SDS-PAGE.
Recombinant Mouse Siglec-15/CD33L3 Protein with Fc tag is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Arg24-Thr262) of mouse Siglec-15/CD33L3 (Accession #NP_001094508.1) fused with a Fc tag at the C-terminus.
100 μgRecombinant Mouse Siglec-15/CD33L3 Protein with His tag, His Tag [orb1550694]
> 90% by SDS-PAGE.
Recombinant Mouse Siglec-15/CD33L3 Protein with His Tag is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Arg24-Thr262) of mouse Siglec-15/CD33L3 (Accession #NP_001094508.1) fused with a 6��His Tag at the C-terminus.
50 μgSiglec-15 Protein (Primary Amine Labeling), Mouse, Recombinant (His), Biotinylated [orb1960652]
98.00%
26.7 kDa (predicted). Due to glycosylation, the protein migrates to 35-43 kDa based on Tris-Bis PAGE result.
1 mg, 500 μg, 100 μg
