SIAE Antibody

• Catalog Number: orb1260531
• Category: Antibodies
• Description: SIAE Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 270-450 of human SIAE (NP_733746.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 58kDa
• Target: SIAE
• UniProt ID: Q9HAT2
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: AIS6, CSE-C, CSEC, LSE, YSG2, sialate O-acetyleste
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SIAE antibody (orb1260531) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.