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Catalog Number | orb1261051 |
---|---|
Category | Antibodies |
Description | SHMT2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 52kDa |
Target | SHMT2 |
UniProt ID | P34897 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | SHMT2, GLYA, SHMT, HEL-S-51e Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using SHMT2 antibody (orb1261051) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded rat kidney using SHMT2 Antibody (orb1261051) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SHMT2 Antibody (orb1261051) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SHMT2 Antibody (orb1261051) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SHMT2 antibody (orb1261051). Blue: DAPI for nuclear staining.
ICC, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Canine, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Canine, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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