SHIP-1 antibody

• Catalog Number: orb44487
• Category: Antibodies
• Description: Mouse Monoclonal to SHIP-1.
• Clonality: Monoclonal
• Clone Number: SHIP-02
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Intracellular staining; recommended dilution: 2-5 μg/ml; positive control: human blood leukocytes. Western blotting: Positive control: RAMOS human cell line, reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: SHIP1
• Entrez: 3635
• UniProt ID: Q92835
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: SHIP-1
• Note: For research use only
• Application notes: Flow cytometry: Intracellular staining; recommended dilution: 2-5 μg/ml; positive control: human blood leukocytes. Western blotting: Positive control: RAMOS human cell line, reducing conditions.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis (reducing conditions) of human SHIP-1 in whole cell lysate of THP-1 human acute monocytic leukemia cell line. Lane 1: immunostaining with Isotype mouse IgG1 control (PPV-04; orb43541); Lane 2, 3: immunostaining with anti-human SHIP-1 (SHIP-01; cat. no. orb44486); Lane 4, 5: immunostaining with anti-human SHIP-1 (SHIP-02).

Separation of MOLT-4 cells stained using anti-SHIP1 (SHIP-02) purified antibody (concentration in sample 3 µg/ml, GAM APC, red-filled) from MOLT-4 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).