Sheep Prolactin Protein

• Catalog Number: orb427876
• Category: Proteins
• Description: Recombinant of sheep Prolactin protein
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Buffer/Preservatives: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.
• Purity: Greater than 99.0% as determined by:(a) Gel filtration analysis.(b) Analysis by SDS-PAGE.
• Protein Sequence: The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro
• Application notes: Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.93 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC DNAman computer analysis program of protein sequences
• Source: Escherichia Coli
• Biological Activity: Is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
• Solubility (25°C): It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H2O or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Stability: Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
• Alternative names: Mammotropin, Luteotropic hormone, Luteotropin, PRL
• Note: For research use only
• Expiration Date: 6 months from date of receipt.