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Product Overview
Product Name Sheep Leptin tA protein
Catalog Number orb426823
ReactivitySheep
ConjugationUnconjugated
Target Leptin tA
Product Properties
Form/Appearance White lyophilized (freeze-dried) powder.
The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Note For research use only.
Protein Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg.
Purity >98.0% as determined by:(a) Gel filtration analysis.(b) Analysis by SDS-PAGE.
Source Escherichia coli.
Activity Biorbyt'sLeptin-Antagonist Triple Mutant Ovine Recombinant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of mouse leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
Product Description

Recombinant of sheep Leptin tA protein

Solubility (25°C)
Solubility (25°C) It is recommended to reconstitute the lyophilized Leptin-Antagonist Triple Mutant Ovine Recombinant in sterile water or sterile 0.4% NaHCO3adjusted to pH 8, not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
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