SHC/SHC1 Antibody

• Catalog Number: orb97023
• Category: Antibodies
• Description: SHC/SHC1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, IHC-Fr, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SHC (424-440aa DPSYVNVQNLDKARQAV), different from the related rat and mouse sequences by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunocytochemistry , 0.5-1μg/ml, Human, -Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, MouseImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By HeatWestern blot, 0.1-0.5μg/ml, Human, Rat, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 62822 MW
• UniProt ID: P29353
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: SHC-transforming protein 1;SHC-transforming protei
• Note: For research use only
• Application notes: WB: The detection limit for SHC1 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Intestine Tissue using SHC1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Brain Tissue using SHC1 antibody

Western blot analysis of Lane 1: Recombinant Human SHC1 Protein 10ng, Lane 2: Recombinant Human SHC1 Protein 5ng, Lane 3: Recombinant Human SHC1 Protein 2.5ng using ERBB4 antibodySHC1 antibody

Western blot analysis of Lane 1: Rat Brain Tissue Lysate, Lane 2: A549 Cell Lysate, Lane 3: A431 Cell Lysate, Lane 4: 293T Cell Lysate, Lane 5: HELA Cell Lysate, Lane 6: JURKAT Cell Lysate using SHC1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Brain Tissue using SHC1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Lung Cancer Tissue using SHC1 antibody