SHC/SHC1 Antibody

• Catalog Number: orb251574
• Category: Antibodies
• Description: SHC/SHC1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IHC-Fr, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SHC (536-564aa VDPEGVVRTKDHRFESVSHLISYHMDNHL), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 62822 MW
• UniProt ID: P29353
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: SHC-transforming protein 1;SHC-transforming protei
• Note: For research use only
• Application notes: WB: The detection limit for SHC is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of SHC using anti-SHC antibody.Lane 1:human A431 Cell;2:human HeLa Cell;3:human HepG2 Cell;4:human Jurkat Cell;5:human K562 Cell;6:human THP-1 Cell;7:rat C6 Cell;8:mouse thymus Tissue;9:mouse RAW246.7 Cell;10:mouse NIH3T3 Cell.

IHC analysis of SHC1 using anti-SHC1 antibody.SHC1 was detected in paraffin-embedded section of Mouse Brain Tissue.

IHC analysis of SHC1 using anti-SHC1 antibody.SHC1 was detected in paraffin-embedded section of Rat Brain Tissue.

IHC analysis of SHC1 using anti-SHC1 antibody.SHC1 was detected in paraffin-embedded section of Human Lung Cancer Tissue.

IHC analysis of SHC1 using anti-SHC1 antibody.SHC1 was detected in frozen section of Human Placenta Tissue.