SHBG Rabbit Polyclonal Antibody

SKU: orb13231

Description

SHBG Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cell Biology, Hormone Research, Lipid Metabolism

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SHBG (51-150/402aa)
• Target: SHBG
• Molecular Weight: 40 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Androgen binding protein; ABP; MGC126834; MGC138391; SBP; Sex steroid binding protein; SHBG; TEBG; Testis specific androgen binding protein; Testosterone binding beta globulin; Testosterone Estradiol Binding Globulin; Testosterone estrogen binding globulin; UniProtKB/Swiss-Prot: P04278.2; SHBG_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (rat liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (orb13231) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (orb13231) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (orb13231) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human U87MG cell lysates, Primary: Anti-SHBG (orb13231) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kDa, Observed band size: 50 kDa.

Sample: Lane 1: Testis (Mouse) Lysate at 30 ug, Lane 2: Liver (Mouse) Lysate at 30 ug, Primary: Anti-ABP/SHBG (orb13231) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 40kD, Observed band size: 44kD.

Quick Database Links

Gene Symbol

SHBG

UniProt

P04278