SGCD Antibody

• Catalog Number: orb1248873
• Category: Antibodies
• Description: SGCD Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: C-RLPHGSYTPTGTR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Human and Rat Heart lysates (calculated MW of 32.2kDa according to NP_000328.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows sarcolemmar staining of the fibres. Recommended concentration, 3-5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SGCD
• UniProt ID: Q92629
• NCBI: NP_758447.1, NP_000328.2, NP_001121681.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SGCD, sarcoglycan, delta (35kDa dystrophin-associa
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Human and Rat Heart lysates (calculated MW of 32.2kDa according to NP_000328.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows sarcolemmar staining of the fibres. Recommended concentration, 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248873 (0.1 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248873 (3.8 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.