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Catalog Number | orb251573 |
---|---|
Category | Antibodies |
Description | SFTPA1/2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IHC-Fr, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SFTPA1/2 (206-237aa VNYTNWYRGEPAGRGKEQCVEMYTDGQWNDRN), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 26182 MW |
UniProt ID | Q8IWL1 |
Sensitivity | > 5000 cells |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Pulmonary surfactant-associated protein A2;PSP-A;P Read more... |
Note | For research use only |
Application notes | WB: The detection limit for SFTPA1/2 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of SFTPA1/2 using anti-SFTPA1/2 antibody.Lane 1:Rat Lung Tissue;2:Mouse Lung Tissue;3:A549 Cell.
IF analysis of SFTPA1/2 using anti-SFTPA1/2 antibody. SFTPA1/2 was detected in a paraffin-embedded section of rat lung tissue.
IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody.SFTPA1/2 was detected in paraffin-embedded section of Mouse Lung Tissue.
IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody.SFTPA1/2 was detected in paraffin-embedded section of Human Lung Cancer Tissue.
IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody.SFTPA1/2 was detected in paraffin-embedded section of Rat Lung Tissue.
IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody.SFTPA1/2 was detected in frozen section of Rat Lung Tissue.
IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody.SFTPA1/2 was detected in frozen section of Mouse Lung Tissue.
IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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