SFRS5 Antibody (Center Y85)

SKU: orb36781

Description

Rabbit polyclonal antibody to SFRS5 in that SR proteins own a characteristic C-terminal Ser/Arg-rich repeat (RS domain) of variable length and constitute a family of highly conserved nuclear phosphoproteins that can function as both essential and alternative pre-mRNA splicing factors. The binding of SR proteins to pre-mRNA appears to be one of the earliest steps in spliceosome assembly, enhancing the binding of U1 snRNP to the 5' splice site to form a commitment complex.

Research Area

Molecular Biology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB: 1:1000, FACS: 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB19850
• Immunogen: Synthetic Peptide
• Target: SRSF5
• Molecular Weight: 31264
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Splicing factor antibody, anti-arginine/serine-rich 5 antibody, anti-Pre-mRNA-splicing factor SRP40 antibody, anti-Delayed-early protein HRS antibody, anti-SRP40 antibody, anti-HRS antibody, anti-SFRS5 antibody

Western blot analysis of SFRS5 antibody (Center Y85) in NCI-H460 cell line lysates (35 ug/lane). SFRS5 (arrow) was detected using the purified Pab.

SFRS5 Antibody (Center Y85) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Quick Database Links

Gene Symbol

SRSF5

UniProt

Q13243

RefSeq (Protein):NP_001034554.1, NP_008856.2