SFN antibody

• Catalog Number: orb19148
• Category: Antibodies
• Description: Goat polyclonal antibody to SFN
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:16000, WB: 0.03-0.1 μg/ml, IHC-P: 2-4µg/ml
• Conjugation: Unconjugated
• MW: 27.8
• Target: 14-3-3 sigma / Stratifin
• Entrez: 2810
• Protein Sequence: DLHTLSEDSYKDST
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SFN, 14-3-3sigma, stratifin, HGNC:10773, YWHAS
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer . Recommended concentration, 2-3ug/ml.WB: Approx. 28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin embedded Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. with SFN antibody.

Western blot analysis of staining of Human Skin lysate (35µg protein in RIPA buffer) using SFN antibody.