14-3-3 sigma/SFN Antibody

• Catalog Number: orb402233
• Category: Antibodies
• Description: 14-3-3 sigma/SFN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells Immunofluorescence, 2μg/ml Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 28 kDa
• UniProt ID: P31947
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: 14-3-3 protein sigma; Epithelial cell marker prote
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-14-3-3 SIGMA antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody.Lane 1:human A431 cell;2:human HeLa cell;3:human MCF-7 cell;4:human Hacat cell;5:rat skin tissue;6:mouse Hepa1-6 cell.

IF analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody.14-3-3 sigma was detected in paraffin-embedded section of A431 cell.

IF analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody.14-3-3 sigma was detected in paraffin-embedded section of A549 cell.

IHC analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody.14-3-3 sigma was detected in paraffin-embedded section of human rectal cancer tissues.