SF3B1 Antibody

• Catalog Number: orb1268788
• Category: Antibodies
• Description: SF3B1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Xenopus
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This SF3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-402 amino acids from the N-terminal region of human SF3B1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 146 kDa
• Target: SF3B1
• UniProt ID: O75533
• NCBI: O75533
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Splicing factor 3B subunit 1, Pre-mRNA-splicing fa
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution Lane 1: PANC-1 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: H-4-Ⅱ-E whole cell lysate Lane 6: A431 whole cell lysate Lane 7: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in CEM cell line lysates (35 ug/lane).

SF3B1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.