Sestrin-1 Antibody

• Catalog Number: orb1263040
• Category: Antibodies
• Description: Sestrin-1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Monkey, Xenopus
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This Sestrin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-341 amino acids from the C-terminal region of human Sestrin-1.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 57 kDa
• Target: SESN1
• UniProt ID: Q9Y6P5
• NCBI: Q9Y6P5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Sestrin-1, p53-regulated protein PA26, SESN1, PA26
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Sestrin-1 antibody in mouse heart tissue lysates (35 ug/lane)

Western blot analysis in NCI-H460, HL-60 cell line lysates (35 ug/lane).This demonstrates the Sestrin-1 antibody detected the Sestrin-1 protein (arrow).

Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.