Sertad1 Rabbit Polyclonal Antibody

SKU: orb100366

Description

Sertad1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Human, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SEI1 (1-42/236aa)
• Target: SERTAD1
• Molecular Weight: 25 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDK 4 binding protein p34SEI1; CDK4 binding protein p34SEI; CDK4 binding protein p34SEI1; SEI 1; SEI1; SERTA domain containing 1; SERTA domain containing 1 variant; SERTA domain containing protein 1; Sertad 1; Transcriptional regulator interacting with the PHD bromodomain 1; TRIP Br1; SRTD1_HUMAN.

Tissue/cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Sertad1 Polyclonal Antibody, Unconjugated (orb100366) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: Mouse embryos tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Sertad1 Polyclonal Antibody, Unconjugated (orb100366) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SERTAD1

UniProt

Q9UHV2

Jung, Samil et al. Oncogenic function of p34SEI-1 via NEDD4‑1‑mediated PTEN ubiquitination/degradation and activation of the PI3K/AKT pathway Int J Oncol, 43, 1587-1595 (2013)

PubMed: 23970032

Applications

IHC

Reactivity

Human

Wu, Min-Ju et al. KLF10 affects pancreatic function via the SEI-1/p21Cip1 pathway Int J Biochem Cell Biol, 60, 53-59 (2015)

PubMed: 25578559

Applications

IHC

Reactivity

Human