SERPINF2 antibody

• Catalog Number: orb19955
• Category: Antibodies
• Description: Goat polyclonal antibody to SERPINF2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 3-5µg/ml
• Conjugation: Unconjugated
• MW: 54.6; 47.9
• Target: Alpha2-antiplasmin
• Entrez: 5345
• Protein Sequence: KDFLQSLKGFPRGDK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti SERPINF2 antibody, anti serpin peptidase inhi
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Liver shows spotty cytoplasm staining in hepatocytes. Recommended concentration, 3-5ug/ml.WB: Approx. 70kDa band observed in Human Duodenum and Ileum lysates (calculated MW of 54.6kDa according to NP_000925.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, Blood. 2004 May 15;103(10):3783-8.; PMID: 14751930). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19955 (0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19955 (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.