SERPINF2 Antibody

• Catalog Number: orb1247807
• Category: Antibodies
• Description: SERPINF2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: EIA, ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KDFLQSLKGFPRGDK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 70kDa band observed in Human Duodenum and Ileum lysates (calculated MW of 54.6kDa according to NP_000925.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, Blood. 2004 May 15;103(10):3783-8.; PMID: 14751930). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:In paraffin embedded Human Liver shows spotty cytoplasm staining in hepatocytes. Recommended concentration, 3-5ug/ml.Enzyme immunoassay: This product has been successfully used in sandwich-type ELISA as the capturing antibody.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SERPINF2
• UniProt ID: P08697
• NCBI: NP_001159393.1, NP_000925.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SERPINF2, serpin peptidase inhibitor, clade F (alp
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 70kDa band observed in Human Duodenum and Ileum lysates (calculated MW of 54.6kDa according to NP_000925.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, Blood. 2004 May 15;103(10):3783-8.; PMID: 14751930). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:In paraffin embedded Human Liver shows spotty cytoplasm staining in hepatocytes. Recommended concentration, 3-5ug/ml.Enzyme immunoassay: This product has been successfully used in sandwich-type ELISA as the capturing antibody.
• Expiration Date: 12 months from date of receipt.

orb1247807 (0.1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247807 (3.8 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.