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PAWR Rabbit Polyclonal Antibody (Carrier-free)

SKU: orb3107444

Description

The PAWR Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, ICC, IF, and WB applications. This antibody targets the PRKC apoptosis WT1 regulator protein in Human samples.

Research Area

Cell Biology, Signal Transduction

Images & Validation

Tested ApplicationsELISA, FC, ICC, IF, WB
Dilution RangeWestern blot, 0.1-0.25 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg /1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml
ReactivityHuman
Application Notes
For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenE.coli-derived human PAWR recombinant protein (Position: R160-D255). Human PAWR shares 82.3% and 81.2% amino acid (aa) sequence identity with mouse and rat PAWR, respectively.
TargetPRKC apoptosis WT1 regulator protein
Molecular Weight41 kDa
PurificationImmunogen affinity purified.
ConjugationCarrier-free

Storage & Handling

StorageAt -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Par 4; PAR4; PAWR; Prostate apoptosis response 4 protein

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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
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FC
Flow Cytometry
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IF
Immunofluorescence
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ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 470.00
Free Secondary Antibody (20 ul)0/0

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