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Description
Research Area
Signal Transduction
Images & Validation
−
| Tested Applications | ELISA, FC, ICC, IF, IHC, WB |
|---|---|
| Dilution Range | Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | E.coli-derived human PELI2 recombinant protein (Position: D118-D420). Human PELI2 shares 97.4% amino acid (aa) sequence identity with mouse?PELI2. |
| Target | E3 ubiquitin-protein ligase pellino homolog 2 |
| Molecular Weight | 50 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Carrier-free |
Storage & Handling
−| Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PELI2; E3 ubiquitin-protein ligase pellino homolog 2; Pellino-2; RING-type E3 ubiquitin transferase pellino homolog 2
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Quick Database Links
Gene Symbol
E3 ubiquitin-protein ligase pellino homolog 2
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)









