SERCA1 rabbit pAb

• Catalog Number: orb766303
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SERCA1
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Amphibian, Human, Mouse, Porcine, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human ATP2A1. AA range:548-597
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 100
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-ATP2A1 antibody, anti-Sarcoplasmic/endoplasmi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using SERCA1 Polyclonal Antibody diluted at 1:2000.

Western blot analysis of lysates from HT-29, A549, and NIH/3T3 cells, using ATP2A1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).