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Catalog Number | orb768079 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Seprase |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human FAP-1. AA range:331-380 |
Concentration | 1 mg/ml |
Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 90 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-FAP antibody, anti-Seprase antibody, anti-170 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
Western Blot analysis of RAT-MUSCLE 293T cells using Seprase Polyclonal Antibody diluted at 1:2000.
Western blot analysis of 3T3 mouse-kidney 293T lysis using Seprase antibody. Antibody was diluted at 1:2000.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FAP-1 Antibody. The picture on the right is blocked with the synthesized peptide.
FC | |
Bovine, Canine, Equine, Porcine, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
APC/Cy5 |