Semaphorin 3E Antibody

• Catalog Number: orb1246973
• Category: Antibodies
• Description: Semaphorin 3E Antibody
• Target: SEMA3E
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-NGGRYGTTKDYPDD
• UniProt ID: O15041
• Tested applications: ELISA, IHC
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0.03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2kDa according to NP_036563.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Prostate shows membranous staining of secretory cells in the gland. Recommended concentration, 4-6ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SEMA3E, sema domain, immunoglobulin domain (Ig), s
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_036563.1, NP_001171600.1

orb1246973 (4 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.