SEMA3G Rabbit Polyclonal Antibody

• Catalog Number: orb312288
• Category: Antibodies
• Description: SEMA3G Rabbit Polyclonal Antibody
• Target: SEMA3G
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human SEMA3G (701-782/782aa)
• UniProt ID: Q9NS98
• MW: 87 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FLJ00014; MGC119473; Sem2; SEM3G_HUMAN; Sema domai
• Note: For research use only

References

Feng, Shandan et al. Resveratrol Plays Protective Roles on Kidney of Uremic Rats via Activating HSP70 Expression Biomed Res Int, 2020, 2126748 (2020)

PubMed: 32280682

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SEMA3G) Polyclonal Antibody, Unconjugated (orb312288) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human Pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SEMA3G) Polyclonal Antibody, Unconjugated (orb312288) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.