Selenophosphate Synthetase 2 antibody

• Catalog Number: orb344638
• Category: Antibodies
• Description: Selenophosphate Synthetase 2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS2.
• Concentration: 2.3 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was purified by Protein A chromatography from monospecific antiserum. This antibody reacts with mouse SPS2 and shows partial cross-reactivity with SPS1. A BLAST analysis was used to suggest cross-reactivity with SPS2 from human sources based on an 84% homology with the immunizing sequence. Cross-reactivity with SPS2 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P97364
• NCBI: 14717785
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Selenophosphate Synthetase 2 Antibody,
• Note: For research use only
• Application notes: This Protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to SPS2 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NIH3T3 cells lysates using Selenophosphate Synthetase 2 antibody