Securin Rabbit Polyclonal Antibody

SKU: orb13669

Description

Securin Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cell Biology, Cell Cycle, Cohesins, Epigenetics

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Equine, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PTTG1 (101-202/202aa)
• Target: PTTG1
• Molecular Weight: 22-28 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AW555095; C87862; Cut2; EAP 1; EAP1; ESP1 ASSOCIATED PROTEIN 1; Esp1-associated protein; hPTTG; MGC126883; MGC138276; Pds1; Pituitary tumor transforming 1; Pituitary tumor transforming protein 1; Pituitary tumor-transforming 1, isoform CRA_a; Pituitary tumor-transforming 1, isoform CRA_b; Pituitary tumor-transforming gene 1; Pituitary tumor-transforming gene 1 protein; PTTG 1; PTTG; PTTG1; PTTG1 protein; PTTG1_HUMAN; Pttg3; Securin; TUMOR TRANSFORMING 1; Tumor transforming protein 1; TUMOR-TRANSFORMING 1; Tumor-transforming protein 1; TUTR 1; TUTR1.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Securin) Polyclonal Antibody, Unconjugated (orb13669) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Securin) Polyclonal Antibody, Unconjugated (orb13669) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Securin) Polyclonal Antibody, Unconjugated (orb13669) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Protein: human colon carcinoma lysates at 30 ug, Primary: Rabbit Anti-Securin (orb13669) at 1:200, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:5000, ECL excitated the fluorescence, Predicted band size: 23kD, Observed band size: 23kD.

Protein: testis (mouse) lysate at 30 ug, Primary: rabbit Anti-Securin (orb13669) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 28 kD, Observed band size: 28 kD.

Quick Database Links

Gene Symbol

PTTG1

UniProt

O95997