Secretogranin V Rabbit Polyclonal Antibody

SKU: orb221892

Description

Secretogranin V Rabbit Polyclonal Antibody

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Secretogranin V (1-100/212aa)
• Target: SCG5
• Molecular Weight: 17/21 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

7B2; 7B2 protein; APPG; Neuroendocrine protein 7B2; P7B2; Pituitary polypeptide; Pituitary polypeptide; Prohormone convertase chaperone; SCG 5; SCG5; Secretogranin 5; Secretogranin V 7B2 protein; Secretogranin5; SecretograninV; Secretory granule endocrine protein I; Secretory granule endocrine protein I; Secretory granule neuroendocrine protein 1 7B2 protein; Secretory granule neuroendocrine protein 1; Sg V; SGNE 1; SGNE1; SgV; 7B2_HUMAN.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-Secretogranin V (orb221892) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17/21 kD, Observed band size: 21 kD.

Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Secretogranin V) Polyclonal Antibody, Unconjugated t 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Protein: spleen (mouse) lysate at 40 ug, Primary: rabbit Anti-Secretogranin V (orb221892) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 17/21 kD, Observed band size: 21 kD.

Quick Database Links

Gene Symbol

SCG5

UniProt

P05408