SDHD Rabbit Polyclonal Antibody

• Catalog Number: orb6907
• Category: Antibodies
• Description: SDHD Rabbit Polyclonal Antibody
• Target: SDHD
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Equine, Gallus, Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human SDHD (81-159/159aa)
• UniProt ID: O14521
• RRID: AB_10927858
• MW: 11 kDa
• Tested applications: ELISA, WB
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CBT1; CII 4; CII-4; CII4; CybS; DHSD_HUMAN; mitoch
• Note: For research use only

References

Mo, Yulin et al. TXNIP contributes to bone loss via promoting the mitochondrial oxidative phosphorylation during glucocorticoid-induced osteoporosis Life Sci, 266, 118938 (2021)

PubMed: 33347878

Applications

WB

Reactivity

Rat

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Heart tissue lysates, Lane 3: Rat Kidney tissue lysates, Primary: Anti-SDHD at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11 kDa, Observed band size: 11 kDa.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-SDHD Polyclonal Antibody, Unconjugated (orb6907) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.