SDHD Antibody

• Catalog Number: orb1269774
• Category: Antibodies
• Description: SDHD Antibody
• Target: SDHD
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This SDHD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human SDHD.
• UniProt ID: O14521
• MW: 17 kDa
• Tested applications: FC, IF, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Succinate dehydrogenase [ubiquinone] cytochrome b
• Note: For research use only
• NCBI: O14521

Western Blot at 1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in Hela cell line lysates (35 ug/lane).

SDHD Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of SDHD Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.