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Catalog Number | orb1824504 |
---|---|
Category | Antibodies |
Description | Succinate dehydrogenase (SDH) is Complex II in the mitochondria, vital for mitochondrial electron transport, as well as Krebs cycle function. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. The SDH complex functions as a tumor suppressor. Loss of any subunit proteins lead to destabilization of the complex and tumor formation. Antibody to SDHB is helpful in the identification of phaeochromocytomas, paragangliomas and GIST. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | SDHB/3745 |
Tested applications | IF, IHC-P |
Reactivity | Human |
Isotype | Mouse IgG2, kappa |
Immunogen | A recombinant human SDHB protein fragment (within amino acids 165-273) was used as the immunogen for the SDHB antibody. |
Antibody Type | Primary Antibody |
Dilution range | Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This SDHB antibody is available for research use only. |
UniProt ID | P21912 |
Storage | Aliquot the SDHB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human liver tissue with SDHB antibody (clone SDHB/3745). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free SDHB antibody (clone SDHB/3745) as confirmation of integrity and purity.
IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |