SDHB Antibody

• Catalog Number: orb2637371
• Category: Antibodies
• Description: Succinate dehydrogenase (SDH) is Complex II in the mitochondria, vital for mitochondrial electron transport, as well as Krebs cycle function.Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. The SDH complex functions as a tumor suppressor. Loss of any subunit proteins lead to destabilization of the complex and tumor formation.Antibody to SDHB is helpful in the identification of phaeochromocytomas, paragangliomas and GIST.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SDHB/2126
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 165-273 was used as the immunogen for the SDHB antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This SDHB antibody is available for research use only.
• UniProt ID: P21912
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the SDHB antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 1) 293, 2) Jurkat and 3) HepG2 cell lysate using SDHB antibody (clone SDHB/2126).

IHC staining of FFPE human kidney tissue with SDHB antibody (clone SDHB/2126). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free SDHB antibody (SDHB/2126) as confirmation of integrity and purity.