SDF4 Antibody

• Catalog Number: orb1249242
• Category: Antibodies
• Description: SDF4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EYKVKFLASKGHSEK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_057260.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse for this particular batch.Immunohistochemistry:In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration: 5-10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SDF4
• UniProt ID: Q9BRK5
• NCBI: NP_057260.2, NP_057631.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SDF4, stromal cell derived factor 4, Cab45, 45 kDa
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_057260.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse for this particular batch.Immunohistochemistry:In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration: 5-10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249242 (0.1 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249242 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.