SDF4 Antibody

SKU: orb1249242

Description

SDF4 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_057260.2). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse for this particular batch.Immunohistochemistry:In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration: 5-10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EYKVKFLASKGHSEK
• Target: SDF4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SDF4, stromal cell derived factor 4, Cab45, 45 kDa calcium-binding protein, OTTHUMP00000001754, SDF-4, calcium binding protein, stromal cell-derived factor 4

orb1249242 (0.1 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249242 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

SDF4

UniProt

Q9BRK5

RefSeq (Protein):NP_057631.1, NP_057260.2