SCXA Antibody / Scleraxis

• Catalog Number: orb1151601
• Category: Antibodies
• Description: Mouse monoclonal antibody to SCXA
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein A/G affinity
• Immunogen: Recombinant full-length human Basic helix-loop-helix transcription factor scleraxis protein was used as the immunogen for the SCXA antibody.
• UniProt ID: Q7RTU7
• Tested applications: FACS, IF
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Application notes: Optimal dilution of the SCXA antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PCRP-SCXA-2D11
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Aliquot the SCXA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This SCXA antibody is available for research use only.
• Note: For research use only

Immunofluorescent staining of human HeLa cells using SCXA antibody (green, clone PCRP-SCXA-2D11) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with SCXA antibody (blue, clone PCRP-SCXA-2D11), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using SCXA antibody (clone PCRP-SCXA-2D11). These results demonstrate the foremost specificity of the PCRP-SCXA-2D11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.