Sclerostin antibody

• Catalog Number: orb100911
• Category: Antibodies
• Description: Sclerostin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Sclerostin. Please contact us for the exact immunogen sequence. The peptide is available as orb374806.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: IHC-P: 1:100-300, WB: 1:200-1000
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 24 kDa
• Target: Sclerostin
• Entrez: 50964
• UniProt ID: Q99P67, Q9BQB4, Q99P68
• NCBI: 21, 079513, 025237, 11
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: Anti BEER antibody, anti CDD antibody, anti Sclero
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Fernandes, Fernanda et al. Oxytocin and bone quality in the femoral neck of rats in periestropause Sci Rep, 10, 7937 (2020)

PubMed: 32404873

Applications

IHC

Reactivity

Rat

WB analysis of human thyroid cancer (lane 1), human endometrial cancer (lane 2), human mammary fibroma (lane 3), human breast cancer (lane 4) using Sclerostin antibody (1 ug/ml)

WB analysis of human thyroid cancer (lane 1), human endometrial cancer (lane 2), human mammary fibroma (lane 3), human breast cancer (lane 4) using Sclerostin antibody (1 ug/ml)

Western blot analysis of rat kidney (lane 1), mouse liver (lane 2), mouse lung (lane 3), mouse spinal cord (lane 4), hela cells (lane 5), human breast cancer (lane 6) using Sclerostin antibody (1 ug/ml)

Immunohistochemical staining of mouse lung tissue using anti-Sclerostin (2.5 ug/ml)

IHC-P staining of mouse bone marrow tissue using Sclerostin antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse bone marrow tissue using anti-Sclerostin (2.5 ug/ml)

Western blot analysis of rat kidney (lane 1), mouse liver (lane 2), mouse lung (lane 3), mouse spinal cord (lane 4), hela cells (lane 5), human breast cancer (lane 6) using Sclerostin antibody (1 ug/ml)

IHC-P image of mouse lung tissue using anti-Sclerostin (2.5 ug/ml)