SCGB1A1 Rabbit Polyclonal Antibody

SKU: orb578101

Description

Rabbit polyclonal antibody to SCGB1A1

Research Area

Disease Biomarkers, Epigenetics & Chromatin, Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SCGB1A1
• Target: SCGB1A1
• Protein Sequence: Synthetic peptide located within the following region: MDTPSSYEAAMELFSPDQDMREAGAQLKKLVDTLPQKPRESIIKLMEKIA
• Molecular Weight: 10kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

UGB, UP1, CC10, CC16, CCSP, UP-1, CCPBP

Sample Tissue: Human HCT15 Whole Cell, Antibody Dilution: 1 ug/ml.

Sample Type: Human Thyroid, Antibody Dilution: 2.0 ug/ml.

Immunohistochemistry with Lung, respiratory epithelium tissue at an antibody concentration of 5 ug/ml using anti-SCGB1A1 antibody (orb578101).

Rabbit Anti-SCGB1A1 Antibody, Catalog Number: orb578101, Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue, Observed Staining: Cytoplasm, Plasma membrane, Primary Antibody Concentration: 1:600, Other Working Concentrations: N/A, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-SCGB1A1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: Human brain.

Quick Database Links

Gene Symbol

SCGB1A1

UniProt

P11684

RefSeq (Protein):NP_003348