SCD Antibody

• Catalog Number: orb1246900
• Category: Antibodies
• Description: SCD Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-RIKRTGDGNYKSG
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Adipose, Brain and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.5kDa according to NP_005054.3. The 30kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Liver and Colon. Recommended concentration: 5ug/ml.Flow Cytometry: Exclusively staining of HepG2 when mixed with Peripheral Blood Lymphocytes.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SCD
• UniProt ID: O00767
• NCBI: NP_005054.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SCD, SCD1, HGNC:10571, FADS5, PRO0998 , acyl-CoA d
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Adipose, Brain and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.5kDa according to NP_005054.3. The 30kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Liver and Colon. Recommended concentration: 5ug/ml.Flow Cytometry: Exclusively staining of HepG2 when mixed with Peripheral Blood Lymphocytes.
• Expiration Date: 12 months from date of receipt.

orb1246900 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246900 (2.5ul) staining of HepG2 cells mixed in with PBL. Detected by fluorescence with PE for SCD in green and with OC515 for CD45 (CYT-450C) in red.

orb1246900 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.