SART3 Antibody

SKU: orb1246982

Description

SART3 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 170kDa band observed in lysates of cell lines Daudi, Jurkat and MOLT4 (calculated MW of 111kDa according to NP_055521.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yang et al, Cancer Res. 1999 Aug 15;59(16):4056-63; PMID: 10463607). Recommended concentration: 0.01-0.03µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-MSNADFAKLFLRK
• Target: SART3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SART3, squamous cell carcinoma antigen recognised by T cells 3, TIP110, KIAA0156, p110(nrb), RP11-13G14, tat-interacting protein of 110 kDa, DSAP1, MGC138188, P100, p110,

orb1246982 staining (0.01 ug/ml) of Jurkat cell lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

SART3

UniProt

Q15020

RefSeq (Protein):NP_055521.1